Fully synthetic platform to rapidly generate tetravalent bispecific nanobody-based immunoglobulins.

ABSTRACT: Nanobodies bind a target antigen with a kinetic profile similar to a conventional antibody, but exist as a single heavy chain domain that can be readily multimerized to engage antigen via multiple interactions. Presently, most nanobodies are produced by immunizing camelids; however, platforms for animal-free production are growing in popularity. Here, we describe the development of a fully synthetic nanobody library based on an engineered human V_H3-23 variable gene and a multispecific antibody-like format designed for biparatopic target engagement. To validate our library, we selected nanobodies against the SARS-CoV-2 receptor-binding domain and employed an on-yeast epitope binning strategy to rapidly map the specificities of the selected nanobodies. We then generated antibody-l

SUBMITTER: Misson Mindrebo L 

PROVIDER: S-EPMC10268213 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature