Genetically engineering endothelial niche in human kidney organoids enables multilineage maturation, vascularization and de novo cell types.

ABSTRACT: Vascularization plays a critical role in organ maturation and cell type development. Drug discovery, organ mimicry, and ultimately transplantation in a clinical setting thereby hinges on achieving robust vascularization of in vitro engineered organs. Here, focusing on human kidney organoids, we overcome this hurdle by combining an inducible ETS translocation variant 2 (ETV2) human induced pluripotent stem cell (iPSC) line, which directs endothelial fate, with a non-transgenic iPSC line in suspension organoid culture. The resulting human kidney organoids show extensive vascularization by endothelial cells with an identity most closely related to endogenous kidney endothelia. Vascularized organoids also show increased maturation of nephron structures including more matur

SUBMITTER: Maggiore JC 

PROVIDER: S-EPMC10274893 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature