Cord blood-derived V_δ2⁺ and V_δ2^- T cells acquire differential cell state compositions upon in vitro expansion.

ABSTRACT: Human cord blood-derived γδ T cells (CB_γδ) display a highly diverse TCR_γδ repertoire and have a unique subtype composition different from fetal or adult peripheral blood counterparts. We expanded CB_γδ in vitro using an irradiated Epstein-Barr virus-transformed feeder cell-based modified rapid expansion protocol (REP). Single-cell RNA sequencing tracked progressive differentiation of naïve CB_γδ into cells expressing neoantigen-reactive tumor-infiltrating lymphocyte as well as tissue-resident memory precursor-like and antigen-presenting cell-like gene signatures. TCR_γδ clonal tracing revealed a bias toward cytotoxic effector differentiation in a much larger proportion of V_δ2^- clones compared to V_δ2^+<

SUBMITTER: Ng JWK 

PROVIDER: S-EPMC10275585 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature