Dataset Information

Detection of Choroidal Neovascularization Using Optical Tissue Transparency.

ABSTRACT:

Purpose

Optical tissue transparency (OTT) provides a tool for visualizing the entire tissue block. This study provides insights into the potential value of OTT with light-sheet fluorescence microscopy (LSFM) in detecting choroidal neovascularization (CNV) lesions.

Methods

OTT with LSFM, hematoxylin and eosin (H&E) staining of paraffin sections, choroidal flatmount immunofluorescence, and optical coherence tomography angiography (OCTA) were used to obtain images of CNV. We determined the rate of change as (Data of week 1 - Data of week 2)/Data of week 1 × 100%. Finally, we compared the rate of change acquired from OTT with LSFM and the other methodologies.

Results

We found that OTT with LSFM can realize three-dimensional (3D) visualizations of the entire CNV. The results showed that the decline in the rate of change from week 1 to week 2 after laser photocoagulation was 33.05% with OTT, 53.01% with H&E staining, 48.11% with choroidal flatmount, 24.06% with OCTA (B-scan), 18.08% with OCTA (en face), 10.98% with OCTA (3D reconstruction), and 7.74% with OCTA (vessel diameter index).

Conclusions

OTT with LSFM will continue to be an invaluable resource for investigators to detect more visualized and quantified information regarding CNV.

Translational relevance

OTT with LSFM now serves as a tool for detecting CNV in mice, and it may undergo human clinical trials in the future.

SUBMITTER: Ma XH

PROVIDER: S-EPMC10278551 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

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Publications

Detection of Choroidal Neovascularization Using Optical Tissue Transparency.

Ma Xiao-Hong XH Feng Wen-Yang WY Xiao Ke K Zhong Zheng Z Fei Peng P Zhao Yin Y Sun Xu-Fang XF

Translational vision science & technology 20230601 6

<h4>Purpose</h4>Optical tissue transparency (OTT) provides a tool for visualizing the entire tissue block. This study provides insights into the potential value of OTT with light-sheet fluorescence microscopy (LSFM) in detecting choroidal neovascularization (CNV) lesions.<h4>Methods</h4>OTT with LSFM, hematoxylin and eosin (H&E) staining of paraffin sections, choroidal flatmount immunofluorescence, and optical coherence tomography angiography (OCTA) were used to obtain images of CNV. We determin ...[more]

PMID: 37318439

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Dataset Information

Detection of Choroidal Neovascularization Using Optical Tissue Transparency.

Purpose

Methods

Results

Conclusions

Translational relevance

Publications

Detection of Choroidal Neovascularization Using Optical Tissue Transparency.

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