Detection of Choroidal Neovascularization Using Optical Tissue Transparency.

ABSTRACT:

Purpose

Optical tissue transparency (OTT) provides a tool for visualizing the entire tissue block. This study provides insights into the potential value of OTT with light-sheet fluorescence microscopy (LSFM) in detecting choroidal neovascularization (CNV) lesions.

Methods

OTT with LSFM, hematoxylin and eosin (H&E) staining of paraffin sections, choroidal flatmount immunofluorescence, and optical coherence tomography angiography (OCTA) were used to obtain images of CNV. We determined the rate of change as (Data of week 1 - Data of week 2)/Data of week 1 × 100%. Finally, we compared the rate of change acquired from OTT with LSFM and the other methodologies.

Results

We found that OTT with LSFM can realize three-dimensional (3D) visualizations of the entire CNV. The resul

SUBMITTER: Ma XH 

PROVIDER: S-EPMC10278551 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature