Dataset Information

Genetic relatedness of third-generation cephalosporin-resistant Escherichia coli among livestock, farmers, and patients in Japan.

ABSTRACT:

Objectives

The third-generation cephalosporin (3GC)-resistant E. coli strains have been detected worldwide in humans and animals. Hence, in this study, we evaluated the prevalence and genetic characteristics of 3GC-resistant E. coli in livestock, farmers, and patients to further analyse if livestock serves as a potential reservoir of antimicrobial-resistant bacteria.

Methods

Faecal samples were collected from 330 healthy livestock (216 cattle and 114 swine), 61 healthy livestock farmers (52 cattle farmers and 9 swine farmers), and 68 non-duplicate 3GC-resistant E. coli isolates were also obtained from the clinical specimens of patients in Japan between 2013 and 2015. Genes associated with resistance in 3GC-resistant E. coli were identified using polymerase chain reaction (PCR) and DNA sequencing. Genotypic diversity was determined by multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE).

Results

We obtained 39 and 17 non-duplicated 3GC-resistant E. coli strains from healthy livestock (33 cattle and six swine) and livestock farmers, respectively. All isolates carried either CTX-M-type extended-spectrum β-lactamase or plasmid-mediated AmpC β-lactamase genes, with CTX-M-14 being the most frequent. CTX-M producers from livestock and patients belonged to 22 and 19 different sequence types (STs), respectively, and only three STs were the same. Among the 3GC-resistant E. coli from livestock and farmers, three types of CTX-M producers have shown similar characteristics (CTX-M genotype, ST, PFGE patterns, and antimicrobial susceptibilities) and were identified as clonal isolates shared among their farms.

Conclusions

Our study findings indicate that CTX-M-14 is predominant in Japan. No distinct relationship was observed between the 3GC-resistant E. coli isolated from livestock and patients; however, some clonal relatedness was observed between the isolates from livestock and farmers due to their close contact.

SUBMITTER: Nakano R

PROVIDER: S-EPMC10288041 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

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Publications

Genetic relatedness of third-generation cephalosporin-resistant <i>Escherichia coli</i> among livestock, farmers, and patients in Japan.

Nakano Ryuichi R Nakano Akiyo A Nishisouzu Ryuji R Hikosaka Kenji K Suzuki Yuki Y Kamoshida Go G Tansho-Nagakawa Shigeru S Endo Shiro S Kasahara Kei K Ono Yasuo Y Yano Hisakazu H

One health (Amsterdam, Netherlands) 20230309

<h4>Objectives</h4>The third-generation cephalosporin (3GC)-resistant <i>E. coli</i> strains have been detected worldwide in humans and animals. Hence, in this study, we evaluated the prevalence and genetic characteristics of 3GC-resistant <i>E. coli</i> in livestock, farmers, and patients to further analyse if livestock serves as a potential reservoir of antimicrobial-resistant bacteria.<h4>Methods</h4>Faecal samples were collected from 330 healthy livestock (216 cattle and 114 swine), 61 healt ...[more]

PMID: 37363218

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