Capillary electrophoresis-mass spectrometry as a tool for Caenorhabditis elegans metabolomics research.

ABSTRACT:

Introduction

Polar metabolites in Caenorhabditis elegans (C. elegans) have predominantly been analyzed using hydrophilic interaction liquid chromatography coupled to mass spectrometry (HILIC-MS). Capillary electrophoresis coupled to mass spectrometry (CE-MS) represents another complementary analytical platform suitable for polar and charged analytes.

Objective

We compared CE-MS and HILIC-MS for the analysis of a set of 60 reference standards relevant for C. elegans and specifically investigated the strengths of CE separation. Furthermore, we employed CE-MS as a complementary analytical approach to study polar metabolites in C. elegans samples, particularly in the context of longevity, in order to address a different part of its metabolome.

Method

We analyzed 60 refere

SUBMITTER: Salzer L 

PROVIDER: S-EPMC10289983 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature