Dataset Information

Capillary electrophoresis-mass spectrometry as a tool for Caenorhabditis elegans metabolomics research.

ABSTRACT:

Introduction

Polar metabolites in Caenorhabditis elegans (C. elegans) have predominantly been analyzed using hydrophilic interaction liquid chromatography coupled to mass spectrometry (HILIC-MS). Capillary electrophoresis coupled to mass spectrometry (CE-MS) represents another complementary analytical platform suitable for polar and charged analytes.

Objective

We compared CE-MS and HILIC-MS for the analysis of a set of 60 reference standards relevant for C. elegans and specifically investigated the strengths of CE separation. Furthermore, we employed CE-MS as a complementary analytical approach to study polar metabolites in C. elegans samples, particularly in the context of longevity, in order to address a different part of its metabolome.

Method

We analyzed 60 reference standards as well as metabolite extracts from C. elegans daf-2 loss-of-function mutants and wild-type (WT) samples using HILIC-MS and CE-MS employing a Q-ToF-MS instrument.

Results

CE separations showed narrower peak widths and a better linearity of the estimated response function across different concentrations which is linked to less saturation of the MS signals. Additionally, CE exhibited a distinct selectivity in the separation of compounds compared to HILIC-MS, providing complementary information for the analysis of the target compounds. Analysis of C. elegans metabolites of daf-2 mutants and WT samples revealed significant alterations in shared metabolites identified through HILIC-MS, as well as the presence of distinct metabolites.

Conclusion

CE-MS was successfully applied in C. elegans metabolomics, being able to recover known as well as identify novel putative biomarkers of longevity.

SUBMITTER: Salzer L

PROVIDER: S-EPMC10289983 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

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Publications

Capillary electrophoresis-mass spectrometry as a tool for Caenorhabditis elegans metabolomics research.

Salzer Liesa L Schmitt-Kopplin Philippe P Witting Michael M

Metabolomics : Official journal of the Metabolomic Society 20230623 7

<h4>Introduction</h4>Polar metabolites in Caenorhabditis elegans (C. elegans) have predominantly been analyzed using hydrophilic interaction liquid chromatography coupled to mass spectrometry (HILIC-MS). Capillary electrophoresis coupled to mass spectrometry (CE-MS) represents another complementary analytical platform suitable for polar and charged analytes.<h4>Objective</h4>We compared CE-MS and HILIC-MS for the analysis of a set of 60 reference standards relevant for C. elegans and specificall ...[more]

PMID: 37351740

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Dataset Information

Capillary electrophoresis-mass spectrometry as a tool for Caenorhabditis elegans metabolomics research.

Introduction

Objective

Method

Results

Conclusion

Publications

Capillary electrophoresis-mass spectrometry as a tool for Caenorhabditis elegans metabolomics research.

Similar Datasets

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