Whole Exome Sequencing to Find Candidate Variants for the Prediction of Kidney Transplantation Efficacy.

ABSTRACT:

Introduction

Kidney transplantation is the optimal treatment strategy for some end-stage renal disease (ESRD); however, graft survival and the success of the transplantation depend on several elements, including the genetics of recipients. In this study, we evaluated exon loci variants based on a high-resolution Next Generation Sequencing (NGS) method.

Methods

We evaluated whole-exome sequencing (WES) of transplanted kidney recipients in a prospective study. The study involved a total of 10 patients (5 without a history of rejection and 5 with). About five milliliters of blood were collected for DNA extraction, followed by whole-exome sequencing based on molecular inversion probes (MIPs).

Results

Sequencing and variant filtering identified nine pathogenic variants in

SUBMITTER: Aghamir SMK 

PROVIDER: S-EPMC10298443 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature