Whole-cell modeling of E. coli colonies enables quantification of single-cell heterogeneity in antibiotic responses.

ABSTRACT: Antibiotic resistance poses mounting risks to human health, as current antibiotics are losing efficacy against increasingly resistant pathogenic bacteria. Of particular concern is the emergence of multidrug-resistant strains, which has been rapid among Gram-negative bacteria such as Escherichia coli. A large body of work has established that antibiotic resistance mechanisms depend on phenotypic heterogeneity, which may be mediated by stochastic expression of antibiotic resistance genes. The link between such molecular-level expression and the population levels that result is complex and multi-scale. Therefore, to better understand antibiotic resistance, what is needed are new mechanistic models that reflect single-cell phenotypic dynamics together with population-level heterogeneity, as an

SUBMITTER: Skalnik CJ 

PROVIDER: S-EPMC10310025 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature