Detection of domestic cat hepadnavirus by next-generation sequencing and epidemiological survey in Japan.

ABSTRACT: The novel domestic cat hepadnavirus (DCH), a member of the Hepadnaviridae, was first detected in Australia and has recently been identified in more countries. In this study, we explored the DCH genome using next-generation sequencing of a plasma sample from a cat with a fever of unknown cause. Nucleotide sequence analysis showed the virus to be relatively genetically distant from the first reported DCH in Australia, showing 89% homology. Then we conducted an epidemiological survey by PCR of plasma samples collected from 203 cats that visited a veterinary hospital for diagnosis and treatment. Two of the 203 surveyed cats a were positive for DCH. One of the two positive cases had elevated liver enzymes of unknown etiology, and the other had hepatocellular adenoma. Our study indicated that DC

SUBMITTER: Sakamoto H 

PROVIDER: S-EPMC10315552 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature