De novo genome assembly of the partial homozygous dihaploid potato identified PVY resistance gene (Ry_chc) derived from Solanum chacoense.

ABSTRACT: The isolation of disease resistance genes introduced from wild or related cultivated species is essential for understanding their mechanisms, spectrum and risk of breakdown. To identify target genes not included in reference genomes, genomic sequences with the target locus must be reconstructed. However, de novo assembly approaches of the entire genome, such as those used for constructing reference genomes, are complicated in higher plants. Moreover, in the autotetraploid potato, the heterozygous regions and repetitive structures located around disease resistance gene clusters fragment the genomes into short contigs, making it challenging to identify resistance genes. In this study, we report that a de novo assembly approach of a target gene-specific homozygous dihaploid deve

SUBMITTER: Akai K 

PROVIDER: S-EPMC10316315 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature