NORMSEQ: a tool for evaluation, selection and visualization of RNA-Seq normalization methods.

ABSTRACT: RNA-sequencing has become one of the most used high-throughput approaches to gain knowledge about the expression of all different RNA subpopulations. However, technical artifacts, either introduced during library preparation and/or data analysis, can influence the detected RNA expression levels. A critical step, especially in large and low input datasets or studies, is data normalization, which aims at eliminating the variability in data that is not related to biology. Many normalization methods have been developed, each of them relying on different assumptions, making the selection of the appropriate normalization strategy key to preserve biological information. To address this, we developed NormSeq, a free web-server tool to systematically assess the performance of normalization methods

SUBMITTER: Scheepbouwer C 

PROVIDER: S-EPMC10320083 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature