Hypoxia sensing requires H₂S-dependent persulfidation of olfactory receptor 78.

ABSTRACT: Oxygen (O₂) sensing by the carotid body is critical for maintaining cardiorespiratory homeostasis during hypoxia. Hydrogen sulfide (H₂S) signaling is implicated in carotid body activation by low O₂. Here, we show that persulfidation of olfactory receptor 78 (Olfr78) by H₂S is an integral component of carotid body activation by hypoxia. Hypoxia and H₂S increased persulfidation in carotid body glomus cells and persulfidated cysteine²⁴⁰ in Olfr78 protein in heterologous system. Olfr78 mutants manifest impaired carotid body sensory nerve, glomus cell, and breathing responses to H₂S and hypoxia. Glomus cells are positive for G_Olf, adenylate cyclase 3 (Adcy3) and cyclic nucleotide-gated channel alpha 2

SUBMITTER: Peng YJ 

PROVIDER: S-EPMC10321732 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature