Novel pyrimidine Schiff bases and their selenium-containing nanoparticles as dual inhibitors of CDK1 and tubulin polymerase: design, synthesis, anti-proliferative evaluation, and molecular modelling.

ABSTRACT: Nanotechnology-based strategies can overcome the limitations of conventional cancer therapies. Hence, novel series of pyrimidine Schiff bases (4-9) were employed in the synthesis of selenium nanoparticle forms (4NPs-9NPs). All selenium nano-sized forms exerted greater inhibitions than normal-sized compounds, far exceeding 5-fluorouracil activity. Compound 4 showed effective anti-proliferative activity against MCF-7(IC₅₀ 3.14 ± 0.04 µM), HepG-2(IC₅₀ 1.07 ± 0.03 µM), and A549(IC₅₀ 1.53 ± 0.01 µM) cell lines, while its selenium nanoform 4NPs showed excellent inhibitory effects, with efficacy increased by 96.52%, 96.45%, and 93.86%, respectively. Additionally, 4NPs outperformed 4 in selectivity against the Vero cell line by 4.5-fold. Furthermore, 4NPs exhibited strong inhibition of CDK1(IC₅₀ 0.47 ± 0.3 µM) and tubulin polymerase(IC₅₀ 0.61 ± 0.04 µM), outperforming 4 and being comparable to roscovitine (IC₅₀ 0.27 ± 0.03 µM) and combretastatin-A4(IC₅₀ 0.25 ± 0.01 µM), respectively. Moreover, both 4 and 4NPs arrested the cell cycle at G0/G1 phase and significantly forced the cells towards apoptosis. Molecular docking demonstrated that 4 and 4NPs were able to inhibit CDK1 and tubulin polymerase binding sites.

SUBMITTER: El-Kalyoubi S 

PROVIDER: S-EPMC10324454 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature