The evolutionary mechanism of non-carbapenemase carbapenem-resistant phenotypes in Klebsiella spp.

ABSTRACT: Antibiotic resistance is driven by selection, but the degree to which a bacterial strain's evolutionary history shapes the mechanism and strength of resistance remains an open question. Here, we reconstruct the genetic and evolutionary mechanisms of carbapenem resistance in a clinical isolate of Klebsiella quasipneumoniae. A combination of short- and long-read sequencing, machine learning, and genetic and enzymatic analyses established that this carbapenem-resistant strain carries no carbapenemase-encoding genes. Genetic reconstruction of the resistance phenotype confirmed that two distinct genetic loci are necessary in order for the strain to acquire carbapenem resistance. Experimental evolution of the carbapenem-resistant strains in growth conditions without the antibiotic reveale

SUBMITTER: Rosas NC 

PROVIDER: S-EPMC10325707 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature