Turnover number predictions for kinetically uncharacterized enzymes using machine and deep learning.

ABSTRACT: The turnover number k_cat, a measure of enzyme efficiency, is central to understanding cellular physiology and resource allocation. As experimental k_cat estimates are unavailable for the vast majority of enzymatic reactions, the development of accurate computational prediction methods is highly desirable. However, existing machine learning models are limited to a single, well-studied organism, or they provide inaccurate predictions except for enzymes that are highly similar to proteins in the training set. Here, we present TurNuP, a general and organism-independent model that successfully predicts turnover numbers for natural reactions of wild-type enzymes. We constructed model inputs by representing complete chemical reactions through differential reaction fingerprints and by representing enzymes through a modified and re-trained Transformer Network model for protein sequences. TurNuP outperforms previous models and generalizes well even to enzymes that are not similar to proteins in the training set. Parameterizing metabolic models with TurNuP-predicted k_cat values leads to improved proteome allocation predictions. To provide a powerful and convenient tool for the study of molecular biochemistry and physiology, we implemented a TurNuP web server.

SUBMITTER: Kroll A 

PROVIDER: S-EPMC10338564 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature