Simultaneous Determination of the Size and Shape of Single α-Synuclein Oligomers in Solution.

ABSTRACT: Soluble oligomers of amyloid-forming proteins are implicated as toxic species in the context of several neurodegenerative diseases. Since the size and shape of these oligomers influence their toxicity, their biophysical characterization is essential for a better understanding of the structure-toxicity relationship. Amyloid oligomers are difficult to characterize by conventional approaches due to their heterogeneity in size and shape, their dynamic aggregation process, and their low abundance. This work demonstrates that resistive pulse measurements using polymer-coated solid-state nanopores enable single-particle-level characterization of the size and shape of individual αSyn oligomers in solution within minutes. A comparison of the resulting size distribution with single-particle analysis

SUBMITTER: Awasthi S 

PROVIDER: S-EPMC10339783 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature