Bifidobacterium adolescentis induces Decorin⁺ macrophages via TLR2 to suppress colorectal carcinogenesis.

ABSTRACT:

Background

The interplay between gut microbiota and tumor microenvironment (TME) in the pathogenesis of colorectal cancer (CRC) is largely unknown. Here, we elucidated the functional role of B. adolescentis and its possible mechanism on the manipulation of Decorin⁺ macrophages in colorectal cancer.

Methods

The relative abundance of B. adolescentis in tumor or para-tumor tissue of CRC patients was analyzed. The role of B. adolescentis was explored in the CRC animal models. The single cell-RNA sequencing (scRNA-seq) was used to investigate the myeloid cells subsets in TME. The expression level of TLR2/YAP axis and its downstream Decorin in macrophages were tested by Western blot and qRT-PCR. Knockdown of Decorin in Raw264.7 was performed to investigate the effect of

SUBMITTER: Lin Y 

PROVIDER: S-EPMC10353206 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature