Multiple Fra-1-bound enhancers showing different molecular and functional features can cooperate to repress gene transcription.

ABSTRACT:

Background

How transcription factors (TFs) down-regulate gene expression remains ill-understood, especially when they bind to multiple enhancers contacting the same gene promoter. In particular, it is not known whether they exert similar or significantly different molecular effects at these enhancers.

Results

To address this issue, we used a particularly well-suited study model consisting of the down-regulation of the TGFB2 gene by the TF Fra-1 in Fra-1-overexpressing cancer cells, as Fra-1 binds to multiple enhancers interacting with the TGFB2 promoter. We show that Fra-1 does not repress TGFB2 transcription via reducing RNA Pol II recruitment at the gene promoter but by decreasing the formation of its transcription-initiating form. This is associated with complex long-rang

SUBMITTER: Bejjani F 

PROVIDER: S-EPMC10354941 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature