Histone deacetylase III interactions with BK polyomavirus large tumor antigen may affect protein stability.

ABSTRACT:

Background

Human polyomavirus BK (BKPyV) causes associated nephropathy and contributes to urinary tract cancer development in renal transplant recipients. Large tumor antigen (LT) is an early protein essential in the polyomavirus life cycle. Protein acetylation plays a critical role in regulating protein stability, so this study investigated the acetylation of the BKPyV LT protein.

Methods

The BKPyV LT nucleotide was synthesized, and the protein was expressed by transfection into permissive cells. The BKPyV LT protein was immunoprecipitated and subjected to LC-MS/MS analysis to determine the acetylation residues. The relative lysine was then mutated to arginine in the LT nucleotide and BKPyV genome to analyze the role of LT lysine acetylation in the BKPyV life cycle.

Resu

SUBMITTER: Hsu YH 

PROVIDER: S-EPMC10354968 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature