PE-STOP: A versatile tool for installing nonsense substitutions amenable for precise reversion.

ABSTRACT: The rapid advances in genome editing technologies have revolutionized the study of gene functions in cell or animal models. The recent generation of double-stranded DNA cleavage-independent base editors has been suitably adapted for interrogation of protein-coding genes on the basis of introducing premature stop codons or disabling the start codons. However, such versions of stop/start codon-oriented genetic tools still present limitations on their versatility, base-level precision, and target specificity. Here, we exploit a newly developed prime editor (PE) that differs from base editors by its adoption of a reverse transcriptase activity, which enables incorporation of various types of precise edits templated by a specialized prime editing guide RNA. Based on such a versatile platform, w

SUBMITTER: Song Z 

PROVIDER: S-EPMC10365944 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature