Improved approaches to channel capacity estimation discover compromised GPCR signaling in diverse cancer cells.

ABSTRACT: Intracellular signaling orchestrates an organism's development and functioning and underlies various pathologies, such as cancer, when aberrant. A universal cell signaling characteristic is channel capacity - the measure of how much information a given transmitting system can reliably transduce. Here, we describe improved approaches to quantify GPCR signaling channel capacity in single cells, averaged across cell population. We assess the channel capacity based on distribution of residuals by the cellular response amplitude. We further develop means to handle irregularly responding cancer cells using the integral values of their response to different agonist concentrations. These approaches enabled us to analyze, for the first time, channel capacity in single cancer cells. A universal feat

SUBMITTER: Koval A 

PROVIDER: S-EPMC10368911 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature