Dataset Information

Chukrasia tabularis limonoid plays anti-inflammatory role by regulating NF-κB signaling pathway in lipopolysaccharide-induced macrophages.

ABSTRACT:

Background

Chukrasia tabularisis, a well-known tropical tree native to southeastern China, has anti-inflammatory and antioxidant activities, and contains large amounts of limonoids and triterpenoids.

Objective

The aim of this study was to investigate the potential anti-inflammatory activity of limonoids from C. tabularis on lipopolysaccharide (LPS)-mediated RAW264.7 cells.

Methods and results

Using a bioassay-guided approach, the chemical fraction with high anti-inflammatory activity was found and its chemical constituents were investigated. Phytochemical studies on active extracts resulted in the separation of three novel phragmalin limonoids (1-3), together with two known limonoids (4-5) and 11 tirucallane triterpenes (6-16). The activity of these isolated compounds in the production of nitric oxide (NO) on LPS-reated macrophages was evaluated. Limonoid 2 indicated significant anti-inflammatory activities with IC₅₀ value of 4.58 μM. Limonoid 2 notably inhibited the production of NO, interleukin- 6 and tumor necrosis factor-α on macrophage. Signal transduction and activation of STAT and NF-κB activators were effectively blocked by limonoid 2.

Conclusions

These results indicate that limonoid 2 has an anti-inflammatory effect by the inhibiting JAK2/STAT3, iNOS/eNOS, and NF-κB signaling pathways and regulating inflammatory mediators.

SUBMITTER: Shen J

PROVIDER: S-EPMC10392864 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Publications

<i>Chukrasia tabularis</i> limonoid plays anti-inflammatory role by regulating NF-<i>κ</i>B signaling pathway in lipopolysaccharide-induced macrophages.

Shen Jinhuang J Cao Fan F Huang Zhiyong Z Ma Xinhua X Yang Nana N Zhang Haitao H Zhang Yonghong Y Zhang Zhiqiang Z

Food & nutrition research 20230714

<h4>Background</h4><i>Chukrasia tabularisis,</i> a well-known tropical tree native to southeastern China, has anti-inflammatory and antioxidant activities, and contains large amounts of limonoids and triterpenoids.<h4>Objective</h4>The aim of this study was to investigate the potential anti-inflammatory activity of limonoids from <i>C. tabularis</i> on lipopolysaccharide (LPS)-mediated RAW264.7 cells.<h4>Methods and results</h4>Using a bioassay-guided approach, the chemical fraction with high an ...[more]

PMID: 37533446

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