Dataset Information

Experimental infection of post-weaned pigs with F18-encoding enterotoxigenic and enterotoxigenic/shigatoxigenic Escherichia coli strain isolated from the diarrheic feces in Korea.

ABSTRACT:

Background

In the porcine industry, Escherichia coli (E. coli) infections have been causing post-weaning diarrhea (PWD) and edema disease (ED) for many years. It is classified into pathotypes and serotypes in animals according to virulence factors. Serotyping is performed for O, K, H, and F antigens, essential for discriminating pathogenicity and epidemiology. Furthermore, E. coli strains that produce F18 fimbriae are major sources of ED and PWD associated with Shiga-toxin producing E. coli (STEC) expressing F18ab and enterotoxigenic E. coli (ETEC) expressing F18ac, respectively.

Aim

To investigate the pathogenicity potential and infection characteristics of experimental infection and confirm the pathological features of the Korean STEC/ETEC strains F18ab and F18ac in piglets.

Methods

Three-week-old pigs were randomized into three experimental groups: infected G1 (F18ab), infected G2 (F18ac), and G3 (control). General health status was monitored daily, and pathological changes were evaluated.

Results

Diarrhea occurred in all infected piglets. Pathological changes were only observed in the small intestine and regional lymph nodes. In G1, mucosal necrosis, inflammatory cell infiltration with hemorrhagic lesions, and apoptotic cell death in the tunica media of arterioles in the small intestine were observed. In contrast, the mucosa and epithelium appeared almost intact, with no abnormal vessel lesions in G2.

Conclusion

Both strains, isolated from pigs in Korea, could be infected and did not spread from the alimentary tract to other organs. The pathological features were quite different among the F18 subtypes. The F18ab strain was more virulent than F18ac, and the virulence characteristics of the F18ac strain were more similar to ETEC than STEC.

SUBMITTER: Baek KH

PROVIDER: S-EPMC10399650 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

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Publications

Experimental infection of post-weaned pigs with F18-encoding enterotoxigenic and enterotoxigenic/shigatoxigenic <i>Escherichia coli</i> strain isolated from the diarrheic feces in Korea.

Baek Kang-Hyun KH Tangchang Warisraporn W Choi Eun-Jin EJ Lee Wan-Kyu WK Lee Kyung-Hyun KH Lee Hyun-Kyung HK Byun Jae-Won JW Son Hwa-Young HY

Open veterinary journal 20230607 6

<h4>Background</h4>In the porcine industry<i>, Escherichia coli (E. coli)</i> infections have been causing post-weaning diarrhea (PWD) and edema disease (ED) for many years. It is classified into pathotypes and serotypes in animals according to virulence factors. Serotyping is performed for O, K, H, and F antigens, essential for discriminating pathogenicity and epidemiology. Furthermore, <i>E. coli</i> strains that produce F18 fimbriae are major sources of ED and PWD associated with Shiga-toxin ...[more]

PMID: 37545702

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