Promoting axon regeneration by inhibiting RNA N6-methyladenosine demethylase ALKBH5.

ABSTRACT: A key limiting factor of successful axon regeneration is the intrinsic regenerative ability in both the peripheral nervous system (PNS) and central nervous system (CNS). Previous studies have identified intrinsic regenerative ability regulators that act on gene expression in injured neurons. However, it is less known whether RNA modifications play a role in this process. Here, we systematically screened the functions of all common m⁶A modification-related enzymes in axon regeneration and report ALKBH5, an evolutionarily conserved RNA m⁶A demethylase, as a regulator of axonal regeneration in rodents. In PNS, knockdown of ALKBH5 enhanced sensory axonal regeneration, whereas overexpressing ALKBH5 impaired axonal regeneration in an m⁶A-dependent manner. Mechani

SUBMITTER: Wang D 

PROVIDER: S-EPMC10400074 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature