Evaluating steroid hormone receptor interactions using the live-cell NanoBRET proximity assay.

ABSTRACT: Steroid hormone receptors play a crucial role in the development and characterization of the majority of breast cancers. These receptors canonically function through homodimerization, but physical interactions between different hormone receptors play a key role in cell functions as well. The estrogen receptor (ERα) and progesterone receptor (PR), for example, are involved in a complex set of interactions known as ERα/PR crosstalk. Here, we developed a valuable panel of nuclear receptor expression plasmids specifically for use in NanoBRET assays to assess nuclear receptor homo- and heterodimerization. We demonstrate the utility of this assay system by assessing ERα/PR physical interaction in the context of the endocrine therapy resistance- associated ERα Y537S mutation. We identify a role o

SUBMITTER: Huggins RJ 

PROVIDER: S-EPMC10402027 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature