The mechanisms related to fibroblasts in burn surface

ABSTRACT: Abstract

Purpose

Mesenchymal stem cells (MSCs) can promote burn wound healing, skin appearance, and function recovery by promoting the differentiation and migration of fibroblasts of a wound. The burn environment can activate the autophagy of MSCs. However, it is not clear whether this autophagy can affect the proliferation and migration of fibroblasts.

Methods

In this study, pretreated MSCs with rapamycin and 3‐methyladenine modulated autophagy and co‐cultured with fibroblasts of burn. Cell migration was detected by immunofluorescence chemical staining. Western blot analysis and enzyme‐linked immunosorbent assay were performed to detect 2,3‐Dioxygenase (IDO), cytokine synthesis inhibitory factor 10 (IL‐10), cytokine synthesis inhibitory factor 6 (IL‐6), prostaglandin E2

SUBMITTER: Zhang G 

PROVIDER: S-EPMC10407725 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature