Generation of functional liver sinusoidal endothelial-like cells from human bone marrow-derived mesenchymal stem cells.

ABSTRACT:

Introduction

Liver sinusoidal endothelial cells (LSECs) are specialized vascular endothelial cells that play an important role in the maintenance of biological homeostasis. However, the lack of versatile human LSECs has hindered research on LSECs and development of medical technologies for liver diseases including hemophilia A. In this study, we developed a technique to induce LSEC differentiation from human bone marrow-derived mesenchymal stem cells (BM-MSCs).

Methods

To induce LSECs from human BM-MSCs, cytokines and chemical compounds associated with signaling implicated in LSEC differentiation and liver development were screened. Then LSEC-related genes and proteins expression in the differentiated cells were analyzed by qPCR and flow cytometry analysis, respectively. LSE

SUBMITTER: Mitani S 

PROVIDER: S-EPMC10412721 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature