Improving the Autofluorescence of Lophira alata Woody Cells via the Removal of Extractives.

ABSTRACT: The autofluorescence phenomenon is an inherent characteristic of lignified cells. However, in the case of Lophira alata (L. alata), the autofluorescence is nearly imperceptible during occasional fluorescence observations. The aim of this study is to investigate the mechanism behind the quenching of lignin's autofluorescence in L. alata by conducting associated experiments. Notably, the autofluorescence image of L. alata observed using optical microscopy appears to be quite indistinct. Abundant extractives are found in the longitudinal parenchyma, fibers, and vessels of L. alata. Remarkably, when subjected to a benzene-alcohol extraction treatment, the autofluorescence of L. alata becomes progressively enhanced under a fluorescence microscope. Addit

SUBMITTER: Yu Z 

PROVIDER: S-EPMC10422229 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature