Are slaughterhouse-obtained livers suitable for use in ex vivo perfusion research?

ABSTRACT:

Objectives

The success of the ex vivo machine perfusion of pig livers used for preclinical research depends on organ quality and availability. In this study, we investigated whether livers obtained from slaughterhouses are suitable and equivalent to livers obtained from laboratory pigs.

Methods

Livers were obtained from slaughterhouse pigs stunned by electrocution or CO₂ inhalation and from laboratory pigs. For the latter group, 45 minutes of warm ischemia was mimicked for a subgroup, ensuring a valid comparison with slaughterhouse-derived livers.

Results

Livers from CO₂-stunned pigs showed lower indocyanine green clearance and bile production, higher blood lactate and potassium concentrations, and higher alanine aminotransferase activitie

SUBMITTER: Ruppelt A 

PROVIDER: S-EPMC10422909 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature