Dataset Information

Evaluation of HLA-G 14bp Ins/Del and +3142 C/G Polymorphisms in Type 1 Diabetes among South Indian Population.

ABSTRACT:

Background

Type 1 diabetes (T1D) is a multifactorial autoimmune disease, involving strong genetic components with familial predisposition. Human leukocyte antigen-G (HLA-G) is a non-classical HLA-class I molecule having several immunomodulatory functions. Polymorphisms in HLA-G are associated with several autoimmune diseases including T1D. This study aims to evaluate the association of HLA-G 14bp Ins/Del and +3142 C/G polymorphisms with T1D among the South Indian population.

Methods

The study was performed in a cohort of 123 T1D patients along with their 51 siblings and 126 parents. The association and linkage of HLA-G 14bp Ins/Del and +3142 C/G polymorphisms with T1D were analysed, and transmission disequilibrium test (TDT) was performed.

Results

Significantly increased frequencies of HLA-G 14bp Del/Del genotype (OR = 2.16, p_c = 0.0302) and Del allele (OR = 1.71, p_c = 0.0398) were observed in female patients compared to parents. Higher frequencies of DelDel/GG combined genotype (OR = 4.45, p_c = 0.0049) and Del/G haplotype (OR = 2.91, p_c = 0.0277) were observed in female patients compared to parents. TDT also revealed over-transmission of Del/G haplotype (25T vs 7UT; P = 0.0015) and a strong linkage disequilibrium between the studied polymorphisms.

Conclusion

This familial study shows the association of HLA-G 3'UTR 14bp Ins/Del polymorphism with the risk of T1D among the South Indian population, especially in females.

SUBMITTER: Gunavathy N

PROVIDER: S-EPMC10424110 | biostudies-literature | 2023 May-Jun

REPOSITORIES: biostudies-literature

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Publications

Evaluation of HLA-G 14bp Ins/Del and +3142 C/G Polymorphisms in Type 1 Diabetes among South Indian Population.

Gunavathy Nagarajan N Asirvatham Arthur A Chitra Ayyappan A Jayalakshmi Mariakuttikan M

Indian journal of endocrinology and metabolism 20230501 3

<h4>Background</h4>Type 1 diabetes (T1D) is a multifactorial autoimmune disease, involving strong genetic components with familial predisposition. Human leukocyte antigen-G (HLA-G) is a non-classical HLA-class I molecule having several immunomodulatory functions. Polymorphisms in <i>HLA-G</i> are associated with several autoimmune diseases including T1D. This study aims to evaluate the association of <i>HLA-G</i> 14bp Ins/Del and +3142 C/G polymorphisms with T1D among the South Indian population ...[more]

PMID: 37583409

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Project description:BackgroundHuman leukocyte antigen (HLA)-G is a non-classical HLA molecule with immunomodulant and immunosuppressive functions, involved in transplantation tolerance. HLA-G14bp ins/del polymorphism in exon 8 has been associated with allograft rejection and kidney transplant outcome, with controversial results. We investigated associations of HLA-G14bp ins/del polymorphism on onset of some of the main post-transplant risk factors, like excess body weight, lipid abnormalities, increased fasting plasma glucose. Polymorphisms of cytokines with both immunosuppressive and metabolic effects were also assessed for comparisons and associated analysis.MethodsThe present study involved kidney transplant recipients (n = 173) in which body mass index, cholesterol, triglycerides, fasting plasma glucose were registered in the first years after transplantation and analyzed in association with genotypes. Presence of hypertension and smoking habits, demographic, transplant-related and therapeutic data of patients were also recorded. Polymerase chain reaction, sequence-specific primer amplification and Taqman allelic discrimination techniques were used for genotyping of HLA-G14bp ins/del, interleukin (IL)-10(-1082G > A,-819 T > C,-592A > C), transforming growth factor-β(+ 869 T > C,+915C > G), IL-6(-174G > C), tumor necrosis factor-α(-308G > A) and IL-18(-137G > C,-607C > A). Effects of genotypes on clinical markers at each time point (pre-transplant and 1 to 5 years after transplant) were analyzed using a repeated-measures general linear model analysis; adjustment for potential confounders was performed.ResultsResults showed that HLA-G14bp ins/ins was significantly associated with obesity, in particular after transplantation (3 years, p = 0.002, OR = 4.48, 95% CI:1.76-11.41). Post-transplant body mass index was significantly increased in HLA-G14bp ins/ins carriers (3 and 4 years, p = 0.033 and p = 0.044); effects of HLA-G14bp genotypes on post-transplant BMI were confirmed by using repeated-measures analysis and after controlling for confounding variables. Cytokine genotypes did not associate with the examined factors.ConclusionsThe study of transplanted patients allowed to evidence a potential relationship between post-transplant weight gain and HLA-G14bp ins/del polymorphism, previously involved in rejection for its immunosuppressive/tolerogenic activity. This novel association could widen the knowledge of the role and functions of HLA-G molecules in diseases and transplantation.

Dataset Information

Evaluation of HLA-G 14bp Ins/Del and +3142 C/G Polymorphisms in Type 1 Diabetes among South Indian Population.

Background

Methods

Results

Conclusion

Publications

Evaluation of HLA-G 14bp Ins/Del and +3142 C/G Polymorphisms in Type 1 Diabetes among South Indian Population.

Similar Datasets

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