Fluoride permeation mechanism of the Fluc channel in liposomes revealed by solid-state NMR.

ABSTRACT: Solid-state nuclear magnetic resonance (ssNMR) methods can probe the motions of membrane proteins in liposomes at the atomic level and propel the understanding of biomolecular processes for which static structures cannot provide a satisfactory description. In this work, we report our study on the fluoride channel Fluc-Ec1 in phospholipid bilayers based on ssNMR and molecular dynamics simulations. Previously unidentified fluoride binding sites in the aqueous vestibules were experimentally verified by ¹⁹F-detected ssNMR. One of the two fluoride binding sites in the polar track was identified as a water molecule by ¹H-detected ssNMR. Meanwhile, a dynamic hotspot at loop 1 was observed by comparing the spectra of wild-type Fluc-Ec1 in variant buffer conditions or with its mutants. Therefore, we propose that fluoride conduction in the Fluc channel occurs via a "water-mediated knock-on" permeation mechanism and that loop 1 is a key molecular determinant for channel gating.

SUBMITTER: Zhang J 

PROVIDER: S-EPMC10446490 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature