Clinical-mediated discovery of pyroptosis in CD8⁺ T cell and NK cell reveals melanoma heterogeneity by single-cell and bulk sequence.

ABSTRACT: Histologically, melanoma tissues had fewer positive cells percentage of pyroptosis-related genes (PRGs), GZMA, GSDMB, NLRP1, IL18, and CHMP4A in epidermal than in normal skin. Pyroptosis, a new frontier in cancer, affects the tumor microenvironment and tumor immunotherapy. Nevertheless, the role of pyroptosis remains controversial, which reason is partly due to the heterogeneity of the cellular composition in melanoma. In this study, we present a comprehensive analysis of the single-cell transcriptome landscape of pyroptosis in melanoma specimens. Our findings reveal dysregulation in the expression of PRGs, particularly in immune cells, such as CD8⁺ cells (representing CD8⁺ T cells) and CD57⁺ cells (representing NK cells). Additionally, the immunohistochemi

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC10449777 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature