Auxin Exposure Disrupts Feeding Behavior and Fatty Acid Metabolism in Adult <i>Drosophila</i>.

ABSTRACT: The ease of genetic manipulation in Drosophila melanogaster using the Gal4/UAS system has been beneficial in addressing key biological questions. Current modifications of this methodology to temporally induce transgene expression require temperature changes or exposure to exogenous compounds, both of which have been shown to have detrimental effects on physiological processes. The recently described auxin-inducible gene expression system (AGES) utilizes the plant hormone auxin to induce transgene expression and is proposed to be the least toxic compound for genetic manipulation, with no obvious effects on Drosophila development and survival in one wild-type strain. Here we show that auxin delays larval development in another widely-used fly strain, and that short- and

SUBMITTER: Fleck SA 

PROVIDER: S-EPMC10462055 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature