Ontology highlight
ABSTRACT:
SUBMITTER: Muthukumar G
PROVIDER: S-EPMC10462106 | biostudies-literature | 2023 Aug
REPOSITORIES: biostudies-literature
Muthukumar Gayathri G Stevens Taylor A TA Inglis Alison J AJ Esantsi Theodore K TK Saunders Reuben A RA Schulte Fabian F Voorhees Rebecca M RM Guna Alina A Weissman Jonathan S JS
bioRxiv : the preprint server for biology 20230817
Mitochondrial outer membrane α-helical proteins play critical roles in mitochondrial-cytoplasmic communication, but the rules governing the targeting and insertion of these biophysically diverse substrates remain unknown. Here, we first defined the complement of required mammalian biogenesis machinery through genome-wide CRISPRi screens using topologically distinct membrane proteins. Systematic analysis of nine identified factors across 21 diverse α-helical substrates reveals that these componen ...[more]