PRDX1 Cys52Ser variant alleviates nonalcoholic steatohepatitis by reducing inflammation in mice.

ABSTRACT:

Objective

Peroxiredoxin 1 (PRDX1) is a peroxidase and guards against oxidative stress by scavenging intracellular peroxides, whereas it also has been shown to stimulate inflammatory response by functioning as a chaperone protein. The potential in vivo link between PRDX1's peroxidase activity and its pro-inflammatory activity remains elusive.

Methods

We generated peroxidase-dead PRDX1 variant mice by mutating its peroxidatic cysteine at 52 (Cys52) to serine, here referred to as PRDX1^Cys52Ser. Trx-TrxR-NADPH coupled activity assay was applied to evaluate the peroxidase activity of global PRDX in PRDX1^Cys52Ser variant mice. PRDX1^Cys52Ser mice and their wild-type littermates were subjected to western diet or methionine and choline deficient diet

SUBMITTER: Bai Z 

PROVIDER: S-EPMC10470253 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature