Dynamic evolution of ceftazidime-avibactam resistance due to interchanges between bla_KPC-2 and bla_KPC-145 during treatment of Klebsiella pneumoniae infection.

ABSTRACT:

Background

The emergence of ceftazidime-avibactam (CZA) resistance among carbapenem-resistant Klebsiella pneumoniae (CRKP) is of major concern due to limited therapeutic options.

Methods

In this study, 10 CRKP strains were isolated from different samples of a patient with CRKP infection receiving CZA treatment. Whole-genome sequencing (WGS) and conjugation experiments were performed to determine the transferability of the carbapenem resistance gene.

Results

This infection began with a KPC-2-producing K. pneumoniae (CZA MIC = 2 μg/mL, imipenem MIC ≥ 16 μg/mL). After 20 days of CZA treatment, the strains switched to the amino acid substitution of T263A caused by a novel KPC-producing gene, bla_KPC-145, which restored carbapenem susc

SUBMITTER: Chen Y 

PROVIDER: S-EPMC10476102 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature