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Protocol to study the role of a human nuclear m6A RNA reader on chromatin-associated RNA targets.

ABSTRACT: Here, we present a detailed protocol to study the role of a human nuclear m6A RNA reader, YTHDC1, on chromatin-associated RNA targets. We describe steps for RNA extraction coupled to subnuclear fractionation to identify and study RNA-based regulations that take place in the chromatin-associated fraction. We then detail an RNA immunoprecipitation procedure adapted to identify chromatin-associated RNA targets. This protocol can be adapted to other human or mammalian chromatin-associated RNA binding proteins. For complete details on the use and execution of this protocol, please refer to Timcheva et al.¹.

SUBMITTER: Seigneurin-Berny D

PROVIDER: S-EPMC10477737 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature

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Protocol to study the role of a human nuclear m6A RNA reader on chromatin-associated RNA targets.

Seigneurin-Berny Daphné D Touat-Todeschini Leila L Timcheva Kalina K Verdel André A

STAR protocols 20230825 3

Here, we present a detailed protocol to study the role of a human nuclear m6A RNA reader, YTHDC1, on chromatin-associated RNA targets. We describe steps for RNA extraction coupled to subnuclear fractionation to identify and study RNA-based regulations that take place in the chromatin-associated fraction. We then detail an RNA immunoprecipitation procedure adapted to identify chromatin-associated RNA targets. This protocol can be adapted to other human or mammalian chromatin-associated RNA bindin ...[more]

PMID: 37632746

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