Neutrophil metalloproteinase driven spleen damage hampers infection control of trypanosomiasis.

ABSTRACT: Recent blood transcriptomic analysis of rhodesiense sleeping sickness patients has revealed that neutrophil signature genes and activation markers constitute the top indicators of trypanosomiasis-associated inflammation. Here, we show that Trypanosoma brucei infection results in expansion and differentiation of four splenic neutrophil subpopulations, including Mki67⁺Birc5⁺Gfi1⁺Cebpe⁺ proliferation-competent precursors, two intermediate immature subpopulations and Cebpb⁺Spi1⁺Irf7⁺Mcl1⁺Csf3r⁺ inflammation reprogrammed mature neutrophils. Transcriptomic scRNA-seq profiling identified the largest immature subpopulation by Mmp8/9 positive tertiary granule markers. We confirmed the presence of both

SUBMITTER: Pham HTT 

PROVIDER: S-EPMC10480172 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature