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Sequence-specific targeting of Caenorhabditis elegans C-Ala to the D-loop of tRNAAla.


ABSTRACT: Alanyl-tRNA synthetase retains a conserved prototype structure throughout its biology. Nevertheless, its C-terminal domain (C-Ala) is highly diverged and has been shown to play a role in either tRNA or DNA binding. Interestingly, we discovered that Caenorhabditis elegans cytoplasmic C-Ala (Ce-C-Alac) robustly binds both ligands. How Ce-C-Alac targets its cognate tRNA and whether a similar feature is conserved in its mitochondrial counterpart remain elusive. We show that the N- and C-terminal subdomains of Ce-C-Alac are responsible for DNA and tRNA binding, respectively. Ce-C-Alac specifically recognized the conserved invariant base G18 in the D-loop of tRNAAla through a highly conserved lysine residue, K934. Despite bearing little resemblance to other C-Ala domains, C. elegans mitochondrial C-Ala robustly bound both tRNAAla and DNA and maintained targeting specificity for the D-loop of its cognate tRNA. This study uncovers the underlying mechanism of how C. elegans C-Ala specifically targets the D-loop of tRNAAla.

SUBMITTER: Antika TR 

PROVIDER: S-EPMC10485164 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature

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Sequence-specific targeting of Caenorhabditis elegans C-Ala to the D-loop of tRNA<sup>Ala</sup>.

Antika Titi Rindi TR   Nazilah Kun Rohmatan KR   Chrestella Dea Jolie DJ   Wang Tzu-Ling TL   Tseng Yi-Kuan YK   Wang Sun-Chong SC   Hsu Hsin-Ling HL   Wang Shao-Win SW   Chuang Tsung-Hsien TH   Pan Hung-Chuan HC   Horng Jia-Cherng JC   Wang Chien-Chia CC  

The Journal of biological chemistry 20230809 9


Alanyl-tRNA synthetase retains a conserved prototype structure throughout its biology. Nevertheless, its C-terminal domain (C-Ala) is highly diverged and has been shown to play a role in either tRNA or DNA binding. Interestingly, we discovered that Caenorhabditis elegans cytoplasmic C-Ala (Ce-C-Ala<sub>c</sub>) robustly binds both ligands. How Ce-C-Ala<sub>c</sub> targets its cognate tRNA and whether a similar feature is conserved in its mitochondrial counterpart remain elusive. We show that the  ...[more]

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