Dataset Information

MicroRNA 29a therapy for CEACAM6-expressing lung adenocarcinoma.

ABSTRACT:

Background

Non-coding microRNAs (miRNAs) play critical roles in tumor progression and hold great promise as therapeutic agents for multiple cancers. MicroRNA 29a (miR-29a) is a tumor suppressor miRNA that inhibits cancer cell growth and tumor progression in non-small cell lung cancer. Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6), which plays an important role in lung cancer progression, has been identified as a target of miR-29a. Here, we evaluated the therapeutic efficacy of a peptide vector capable of delivering miR-29a intracellularly using the acidic tumor microenvironment in a lung adenocarcinoma xenograft mouse model.

Methods

A miRNA delivery vector was constructed by tethering the peptide nucleic acid form of miR-29a to a peptide with a low pH-induced transmembrane structure (pHLIP) to enable transport of the miRNAs across the plasma membrane. Tumor suppressive effects of pHLIP-miR29a on lung adenocarcinoma development in vivo were assessed using a BALB/c xenograft model injected with A549 cells.

Results

Incubation of A549 cells with pHLIP-miR-29a at an acidic pH downregulated endogenous CEACAM6 expression and reduced cell viability. Intravenous injection of the mice with pHLIP-miR-29a inhibited tumor growth by up to 18.1%. Intraperitoneal injection of cisplatin reduced tumor volume by 29.9%. Combined pHLIP-miR-29a + cisplatin treatment had an additive effect, reducing tumor volume up to 39.7%.

Conclusions

Delivery of miR-29a to lung adenocarcinoma cells using a pHLIP-mediated method has therapeutic potential as a unique cancer treatment approach.

SUBMITTER: Son SM

PROVIDER: S-EPMC10492333 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature

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Publications

MicroRNA 29a therapy for CEACAM6-expressing lung adenocarcinoma.

Son Seung-Myoung SM Yun Jieun J Kim Dong-Wook DW Jung Young-Suk YS Han Sang-Bae SB Lee Yong Hee YH Han Hye Sook HS Woo Chang Gok CG Lee Ho-Chang HC Lee Ok-Jun OJ

BMC cancer 20230908 1

<h4>Background</h4>Non-coding microRNAs (miRNAs) play critical roles in tumor progression and hold great promise as therapeutic agents for multiple cancers. MicroRNA 29a (miR-29a) is a tumor suppressor miRNA that inhibits cancer cell growth and tumor progression in non-small cell lung cancer. Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6), which plays an important role in lung cancer progression, has been identified as a target of miR-29a. Here, we evaluated the therapeutic ...[more]

PMID: 37684602

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Dataset Information

MicroRNA 29a therapy for CEACAM6-expressing lung adenocarcinoma.

Background

Methods

Results

Conclusions

Publications

MicroRNA 29a therapy for CEACAM6-expressing lung adenocarcinoma.

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