Altered cfDNA fragmentation profile in hypomethylated regions as diagnostic markers in breast cancer.

ABSTRACT:

Background

Breast cancer, the most common malignancy in women worldwide, has been proven to have both altered plasma cell-free DNA (cfDNA) methylation and fragmentation profiles. Nevertheless, simultaneously detecting both of them for breast cancer diagnosis has never been reported. Moreover, although fragmentation pattern of cfDNA is determined by nuclease digestion of chromatin, structure of which may be affected by DNA methylation, whether cfDNA methylation and fragmentation are biologically related or not still remains unclear.

Methods

Improved cfMeDIP-seq were utilized to characterize both cfDNA methylation and fragmentation profiles in 49 plasma samples from both healthy individuals and patients with breast cancer. The feasibility of using cfDNA fragmentation profile i

SUBMITTER: Wang J 

PROVIDER: S-EPMC10517480 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature