Molecular mechanisms of colistin- and multidrug-resistance in bacteria among patients with hospital-acquired infections.

ABSTRACT:

Aim

The increasing burden of resistance in Gram-negative bacteria (GNB) is becoming a major issue for hospital-acquired infections. Therefore, understanding the molecular mechanisms is important.

Methodology

Resistance genes of phenotypically colistin-resistant GNB (n = 60) were determined using whole genome sequencing. Antimicrobial susceptibility patterns were detected by Vitek®2 & broth microdilution.

Results

Of these phenotypically colistin-resistant isolates, 78% were also genetically resistant to colistin. Activation of efflux pumps, and point-mutations in pmrB, and MgrB genes conferred colistin resistance among GNB. Eight different strains of K. pneumoniae were identified and ST43 was the most prominent strain with capsular type-specific (c

SUBMITTER: Khurana S 

PROVIDER: S-EPMC10518808 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature