Highly sensitive and rapid point-of-care testing for HIV-1 infection based on CRISPR-Cas13a system.

ABSTRACT:

Background

Human immunodeficiency virus type one (HIV-1) is the leading cause of acquired immunodeficiency syndrome (AIDS). AIDS remains a global public health concern but can be effectively suppressed by life-long administration of combination antiretroviral therapy. Early detection and diagnosis are two key strategies for the prevention and control of HIV/AIDS. Rapid and accurate point-of-care testing (POCT) provides critical tools for managing HIV-1 epidemic in high-risk areas and populations.

Methods

In this study, a POCT for HIV-1 RNA was developed by CRISPR-Cas13a lateral flow strip combined with reverse transcriptase recombinase-aided amplification (RT-RAA) technology, the results can be directly observed by naked eyes.

Results

Moreover, with the degenerate bas

SUBMITTER: Li X 

PROVIDER: S-EPMC10518925 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature