QSanger: Quantification of Genetic Variants in Bacterial Cultures by Sanger Sequencing.

ABSTRACT: Genetic variations such as mutations and recombinations arise spontaneously in all cultured organisms. Although it is possible to identify nonneutral mutations by selection or counterselection, the identification of neutral mutations in a heterogeneous population usually requires expensive and time-consuming methods such as quantitative or droplet polymerase chain reaction and high-throughput sequencing. Neutral mutations could even become dominant under changing environmental conditions enforcing transitory selection or counterselection. We propose a novel method, which we called qSanger, to quantify DNA using amplitude ratios of aligned electropherogram peaks from mixed Sanger sequencing reads. Plasmids expressing enhanced green fluorescent protein and mCherry fluorescent markers were us

SUBMITTER: Prakash S 

PROVIDER: S-EPMC10521659 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature