Targeting the hedgehog signaling pathway to improve tendon-to-bone integration.

ABSTRACT:

Objective

While the role of hedgehog (Hh) signaling in promoting zonal fibrocartilage production during development is well-established, whether this pathway can be leveraged to improve tendon-to-bone repair in adults is unknown. Our objective was to genetically and pharmacologically stimulate the Hh pathway in cells that give rise to zonal fibrocartilaginous attachments to promote tendon-to-bone integration.

Design

Hh signaling was stimulated genetically via constitutive Smo (SmoM2 construct) activation of bone marrow stromal cells or pharmacologically via systemic agonist delivery to mice following anterior cruciate ligament reconstruction (ACLR). To assess tunnel integration, we measured mineralized fibrocartilage (MFC) formation in these mice 28 days post-surgery and per

SUBMITTER: Kamalitdinov TB 

PROVIDER: S-EPMC10524548 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature