The World's First Acne Dysbiosis-like Model of Human 3D Ex Vivo Sebaceous Gland Colonized with Cutibacterium acnes and Staphylococcus epidermidis.

ABSTRACT: Acne-prone skin is associated with dysbiosis involving Cutibacterium acnes (C. acnes) and Staphylococcus epidermidis (S. epidermidis) causing increased seborrhea in sebaceous glands (SG) and inflammation. Human primary sebocytes were cultivated using 1.10⁶ UFC/mL C. acnes Type IA (facial acne, ATCC6919) and/or 1.10⁵ UFC/mL S. epidermidis (unknown origin, ATCC12228) for 48 h in our SEB4GLN-optimized media without antibiotics. Bacteria and sebocytes were enumerated and assessed to determine their viability. Lipid production was imaged and quantified via Nile Red staining. SG with hair follicles were microdissected from healthy skin and cultured using 1.10⁵ UFC/mL C. acnes Type 1A and/or 1.10⁴ UFC/mL

SUBMITTER: Forraz N 

PROVIDER: S-EPMC10537848 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature