Self-Assembly of DNA Nanostructures in Different Cations.

ABSTRACT: The programmable nature of DNA allows the construction of custom-designed static and dynamic nanostructures, and assembly conditions typically require high concentrations of magnesium ions that restricts their applications. In other solution conditions tested for DNA nanostructure assembly, only a limited set of divalent and monovalent ions are used so far (typically Mg²⁺ and Na⁺ ). Here, we investigate the assembly of DNA nanostructures in a wide variety of ions using nanostructures of different sizes: a double-crossover motif (76 bp), a three-point-star motif (~134 bp), a DNA tetrahedron (534 bp) and a DNA origami triangle (7221 bp). We show successful assembly of a majority of these structures in Ca²⁺ , Ba²⁺ , Na⁺ , K⁺ and Li⁺ and provide quantified assembly yields using gel electrophoresis and visual confirmation of a DNA origami triangle using atomic force microscopy. We further show that structures assembled in monovalent ions (Na⁺ , K⁺ and Li⁺ ) exhibit up to a 10-fold higher nuclease resistance compared to those assembled in divalent ions (Mg²⁺ , Ca²⁺ and Ba²⁺ ). Our work presents new assembly conditions for a wide range of DNA nanostructures with enhanced biostability.

SUBMITTER: Rodriguez A 

PROVIDER: S-EPMC10538431 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature