A periosteum-derived cell line to study the role of BMP/TGFβ signaling in periosteal cell behavior and function.

ABSTRACT: The periosteum is a thin tissue surrounding each skeletal element that contains stem and progenitor cells involved in bone development, postnatal appositional bone growth, load-induced bone formation, and fracture repair. BMP and TGFβ signaling are important for periosteal activity and periosteal cell behavior, but thorough examination of the influence of these pathways on specific cell populations resident in the periosteum is lacking due to limitations associated with primary periosteal cell isolations and in vitro experiments. Here we describe the generation of a novel periosteum-derived clonal cell (PDC) line from postnatal day 14 mice and use it to examine periosteal cell behavior in vitro. PDCs exhibit key characteristics of periosteal cells observed during skeletal dev

SUBMITTER: Moore ER 

PROVIDER: S-EPMC10547901 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature