Diabetes Reshapes the Circadian Transcriptome Profile in Murine Retina.

ABSTRACT:

Purpose

Diabetic retinopathy (DR) is a common complication of diabetes and has a high prevalence. Dysregulation of circadian rhythmicity is associated with the development of DR. This research aimed to investigate rhythmical transcriptome alterations in the retina of diabetic mice.

Methods

C57BL/6J mice were used to establish a diabetes model by intraperitoneal injection of streptozotocin (STZ). After 12 weeks, retinas were collected continuously at 4-hour intervals over 1 day. Total RNA was extracted from normal and STZ-treated retinas and RNA sequencing was performed. Meta2d algorithm, Kyoto Encyclopedia of Genes, Phase Set Enrichment Analysis, and time-series cluster analysis were used to identify, analyze and annotate the composition, phase, and molecular functions of rh

SUBMITTER: Ye S 

PROVIDER: S-EPMC10552875 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature