Comparing the value of mono- vs coculture for high-throughput compound screening in hematological malignancies.

ABSTRACT: Large-scale compound screens are a powerful model system for understanding variability of treatment response and discovering druggable tumor vulnerabilities of hematological malignancies. However, as mostly performed in a monoculture of tumor cells, these assays disregard modulatory effects of the in vivo microenvironment. It is an open question whether and to what extent coculture with bone marrow stromal cells could improve the biological relevance of drug testing assays over monoculture. Here, we established a high-throughput platform to measure ex vivo sensitivity of 108 primary blood cancer samples to 50 drugs in monoculture and coculture with bone marrow stromal cells. Stromal coculture conferred resistance to 52% of compounds in chronic lymphocytic leukemia (CLL) and 36% of compound

SUBMITTER: Herbst SA 

PROVIDER: S-EPMC10558604 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature